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1. Primidone (10 µg/ml); 2. Phenobarbital (10 µg/ml; 3. Phenytoin (20 µg/ml); 4. Carbamazepine (5 µg/ml)

Determination of Anticonvulsant drugs and Methyl Xanthine derivatives in serum

Article number: VPH0013J

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Eurospher 100-5 C18, 150 x 4.6 mm ID

Order No. 15EE181ESJ

Vertex Plus columns, 30x4 mm, 50x4 mm, 100x4 mm, 120x4 mm, 125x4 mm, 150x4 mm

For a link to the column see below: Related products


Details
Method

HPLC

Sample

precolumn loading and washing with Eluent A

Eluent

A: 14 mM NaH2PO4 - 6mM NaH2PO4

B: Eluent A / Acetonitrile / Methanol (6.5:1.5:2)

 

Gradient

isocratic

Flow rate

0.8 ml/min

Mode

RP Mode

Injection volume

20 µl

Temperature

25 °C

Detection

UV at 230 nm

Substances

Primidone, Phenobarbital, Phenytoin, Carbamazepine

Key words

Drugs, Anticonvulsant drugs

Chromatogram

1. Primidone (10 µg/ml)

2. Phenobarbital (10 µg/ml

3. Phenytoin (20 µg/ml)

4. Carbamazepine (5 µg/ml)

 

Downloads
  Title Description Languages
VPH0013J short application Determination of Anticonvulsant drugs and
Methyl Xanthine derivatives in serum
Related products
  Title Article number Description
VertexPlus Column 150 x 4.6 mm Eurospher 100-5 C18 15EE181ESJ VertexPlus Column 150 x 4.6 mm Eurospher 100-5 C18

Technical data are subject to change without notice.

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